Journal: Bioactive Materials

Article Title: Dynamic hydrogels orchestrate the differentiation fate of mesenchymal stem cells through epigenetic regulation of SETD7 to accelerate bone defect repair

doi: 10.1016/j.bioactmat.2026.01.019

Figure Lengend Snippet: SETD7-mediated β-catenin methylation enhances its nuclear accumulation and requires binding to nuclear YAP for osteogenic activity. (a) GO analysis of predicted miRNA targets (top 20 terms). (b) Co-IP of cytoplasmic β-catenin complexes from hMSCs in HA-CA and HA-ADA hydrogels, immunoblotted for SETD7 and methyl-lysine. (c) Nuclear β-catenin and H3 levels. (d, e) Quantification of (b) and (c). (f) Co-IP analysis following SETD7 overexpression (MOI = 20, 12 h transduction, 48 h culture). (g) Nuclear β-catenin levels after SETD7 overexpression. (h, i) Quantification of (f) and (g). (j) Co-IP analysis following SETD7 knockdown (MOI = 10, 24 h transduction, 48 h culture). (k) Nuclear β-catenin levels after SETD7 silencing. (l, m) Quantification of (j) and (k). (n, o) Nuclear YAP and H3 expression with quantification. (p, q) Co-IP analysis of nuclear YAP-β-catenin interaction in hMSCs cultured in HA-CA and HA-ADA hydrogels. (r) β-catenin/YAP co-localization by immunofluorescence. (s) β-catenin and YAP staining after gene silencing. (t, u) ARS staining and quantification of osteogenic differentiation following knockdown. n = 3. Data are presented as mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: After blocking with 5 % non-fat milk for 1 h at room temperature, membranes were incubated with primary antibodies including β-catenin (1:800, Santa Cruz Biotechnology, #sc-7963), YAP (1:1000, ABclonal, #A19134), SETD7 (1:1000, Proteintech, #24840-1-AP), methylated lysine (1:1200, Abcam, #ab23366), Runx2 (1:1000, ABclonal, #A2851), OCN (1:1000, ABclonal, #A20800), and PPARγ (1:1000, ABclonal, #A11183) overnight at 4 °C, followed by HRP-conjugated secondary antibodies for 1 h at room temperature.

Techniques: Methylation, Binding Assay, Activity Assay, Co-Immunoprecipitation Assay, Over Expression, Transduction, Knockdown, Expressing, Cell Culture, Immunofluorescence, Staining

Journal: Bioactive Materials

Article Title: Dynamic hydrogels orchestrate the differentiation fate of mesenchymal stem cells through epigenetic regulation of SETD7 to accelerate bone defect repair

doi: 10.1016/j.bioactmat.2026.01.019

Figure Lengend Snippet: Schematic illustration of the HA-ADA hydrogel system for enhanced bone regeneration through dynamic matrix-mediated mechanotransduction. (a) Experimental workflow. (b) Chemical composition of hydrogels showing HA-ADA or HA-CA polymers decorated with RGD motifs, which crosslink with Ac-β-CD under blue light to form distinct network architectures. (c) Comparative illustration of hydrogel performance in bone regeneration. HA-ADA hydrogels exhibit high network adaptability that enables cell spreading through dynamic “gate opening” mechanisms involving actin polymerization and F-actin bundle formation, leading to enhanced bone regeneration. In contrast, HA-CA hydrogels display low network adaptability with restricted “gate closed” states that limit cell spreading and result in reduced bone regeneration capacity. (d) Molecular mechanism underlying HA-ADA hydrogel-promoted osteogenesis. The dynamic matrix downregulates miR-376a-3p and miR-127-5p expression, thereby relieving their repression of SETD7. Elevated SETD7 subsequently methylates β-catenin, facilitating YAP/β-catenin signaling cascade activation to drive osteogenic differentiation.

Article Snippet: After blocking with 5 % non-fat milk for 1 h at room temperature, membranes were incubated with primary antibodies including β-catenin (1:800, Santa Cruz Biotechnology, #sc-7963), YAP (1:1000, ABclonal, #A19134), SETD7 (1:1000, Proteintech, #24840-1-AP), methylated lysine (1:1200, Abcam, #ab23366), Runx2 (1:1000, ABclonal, #A2851), OCN (1:1000, ABclonal, #A20800), and PPARγ (1:1000, ABclonal, #A11183) overnight at 4 °C, followed by HRP-conjugated secondary antibodies for 1 h at room temperature.

Techniques: Expressing, Activation Assay

Journal: Non-coding RNA Research

Article Title: Exosomal miRNA-218–5p derived from low-passage dermal papilla cells modulates hair follicle growth and development

doi: 10.1016/j.ncrna.2026.01.004

Figure Lengend Snippet: MiR-218-5p regulated HF growth- and development-related gene expression in HFSCs. (A) MiR-218–5p expression levels in HFSCs after transfection with miR-218–5p mimics or the inhibitor (unpaired two-tailed t -test, n = 3). (B) Expression of HF development-related genes in HFSCs is regulated by miR-218–5p. (C) β-Catenin and SFRP2 protein expression in HFSCs after treatment with miR-218–5p mimics or inhibitor (unpaired two-tailed t -test, n = 3). ∗ P < 0.05, ∗∗ P < 0.01.

Article Snippet: Anti-SFRP2 rabbit polyclonal antibody (Proteintech Biotech, Cat No. 12189-1-AP), anti-β-catenin polyclonal antibody (Proteintech, China, Cat No. 51067-2-AP), and anti-GAPDH mouse monoclonal antibody (Proteintech, China, Cat No. 60004-1-Ig) were the primary antibodies.

Techniques: Gene Expression, Expressing, Transfection, Two Tailed Test

Journal: Frontiers in Psychiatry

Article Title: Bioinformatics analysis of the mechanisms and efficacy of the Bushen Anzhi recipe in treating aging-related insomnia

doi: 10.3389/fpsyt.2026.1770410

Figure Lengend Snippet: Behavioral assessments and analysis of Wnt pathway-related protein expression. (A) Water maze trajectory; (B) Open-field trajectory; (C) Time to escape latency; (D) Time in quadrant 4; (E) Times of crossing the platform; (F) Average speed; (G) Times of climbing a wall; (H) GSK-3β content; (I) western blot bands of β-catenin and Wnt3a; (J) The level of β-catenin; (K) The level of Wnt3a. ARI, aging-related insomnia; BSAZ, Bushen Anzhi recipe; ### p < 0.001, Con vs. ARI. * p < 0.05, ** p < 0.01, *** p < 0.001, ARI vs. ARI-BSAZ.

Article Snippet: Samples were added to a pre-cast polyacrylamide gel, electrophoresed in a Running Buffer containing 10% SDS, transferred to PVDF membrane, and Anti-Wnt3a (1: 800, GB153750 , Servicebio, China), Anti-β-catenin(1:1000, GB150016 , Servicebio, China), Anti-β-tubulin(1:1000, GB15140, Servicebio, China) were incubated overnight, and the secondary antibody was added, and the automatic luminometer was used.

Techniques: Expressing, Western Blot

Journal: Cancer Medicine

Article Title: STEAP1 Suppresses Oral Squamous Cell Carcinoma by Targeting Wnt/β‐Catenin Signalling and EMT

doi: 10.1002/cam4.71862

Figure Lengend Snippet: STEAP1 Effect on the wnt/β‐catenin signalling pathway in OSCC cell lines. (A–J) The effect of STEAP1 on the wnt/β‐catenin signalling pathway in SAS and Tca‐8113 cells was assessed and quantitatively analysed by Western Blot. (* p < 0.05, ** p < 0.01, **** p < 0.0001).

Article Snippet: China); a mouse anti‐human β‐actin antibody (TA‐09, ZSBIO, China); a rabbit anti‐human P‐GSK‐3β antibody (BM4837, BOSTER, China); a rabbit anti‐human GSK‐3β antibody (A00791‐3, BOSTER, China); a rabbit anti‐human β‐catenin antibody (PA1212, BOSTER, China); primary rabbit anti‐human c‐Myc antibody ( T55150 , Abmart, China); primary rabbit anti‐human Axin2 antibody ( R23574 , ZENBIO, China); secondary goat anti‐mouse IgG antibody (ZB2305, ZSBIO, China); secondary goat anti‐rabbit IgG antibody (ZB2301, ZSBIO, China).

Techniques: Western Blot